Reference Number: 436
Year: 2018
Link: Link to original paper
Nutrition: Polyphenols | Prebiotic
Summary
Background
Coffee is widely consumed and contains many bioactive compounds, any of which may impact pathways related to disease development.
Objective
To identify individual metabolite changes in response to coffee.
Methods
We profiled the metabolome of fasting serum samples collected from a previously reported single?blinded, three?stage clinical trial. Forty?seven habitual coffee consumers refrained from drinking coffee for 1 month, consumed four cups of coffee/day in the second month and eight cups/day in the third month. Samples collected after each coffee stage were subject to nontargeted metabolomic profiling using UPLC?ESI?MS/MS. A total of 733 metabolites were included for univariate and multivariate analyses.
Results
A total of 115 metabolites were significantly associated with coffee intake (P < 0.05 and Q < 0.05). Eighty?two were of known identity and mapped to one of 33 predefined biological pathways. We observed a significant enrichment of metabolite members of five pathways (P < 0.05): (i) xanthine metabolism: includes caffeine metabolites, (ii) benzoate metabolism: reflects polyphenol metabolite products of gut microbiota metabolism, (iii) steroid: novel but may reflect phytosterol content of coffee, (iv) fatty acid metabolism (acylcholine): novel link to coffee and (v) endocannabinoid: novel link to coffee.
Conclusions
The novel metabolites and candidate pathways we have identified may provide new insight into the mechanisms by which coffee may be exerting its health effects.
Significance of this study:
This shows that in some people, coffee may exert positive health benefits due to its polyphenol content, and the metabolism of these polyphenols by the gut microbiota.